The enzyme phosphoglycerate kinase (PGK) transfer phosphoryl group from 1,3 bisphosphate glycerate to ADP forming ATP and 3-phospholycerate. Glucose is changed to glucose 6 phosphate by glucokinase/ hexokinase in glycolysis, that is totally reversed by glucose 6 phosphatase. Glucose and Fructose both are 6 carbon compounds and are isomers of each other and phosphorous is still attached to 6th carbon. The phosphorous thus released combines with ADP and forms ATP. As 2 ATP’s are used at start, then the NET ATP PRODUCTION=(10-2)=8 ATP’s.                            Â. The  Fructose-1,6-Biphosphate thus formed is unstable and splits into 3 carbon containing compounds called Dihydroxy acetone Phosphate(DHAP) and Glyceraldehyde-3-Phosphate(G3P) in the presence of Aldolase enzyme. Without one enzyme Glycolysis process Stops. After Glycolysis, the presence or absence of oxygen determines the forward process. If oxygen is not available(Anaerobic)-Then, 2 molecules of pyruvic acids enters in fermentation process to form lactate. Enolase promote reversible removal of a molecule of water from 2-phosphoglycerate forming Phosphoenol-pyruvete (PEP). In glioblastoma stem-like cells, hypoxia induces downregulation of pentose phosphate pathway (PPP) enzymes and a flux shift towards glycolysis. The process of glycolysis involves series of 10 reactions which occurs in presence of different catalytic enzymes. That means- Two compounds of same isomers are formed. The glycolytic pathway is a major metabolic pathway for microbial fermentation which involves the catabolism of glucose into pyruvate. The overall reaction of Glycolysis is represented or written as shown below:-, C6H12O6 + 2 NAD+ + 2 ADP + 2 P ➡️ 2(CH3(C=O)COOH + 2 ATP + 2 NADH + 2 H+, ✓NADH=Nicotinamide Adenine Dinucleotide Hydrogen, ✓NAD=Nicotinamide Adenine DinucleotideÂ. Glucose is phosphorylated at –OH group of C6 in which one molecule ATP is consumed. Other enzymes are also equally important. Glycogenolysis is not the reversal of Glycogenesis. Thank you for watching!                         Phosohofructo Kinase, Fructose-6-Phosphate⬅️➡️Fructose-1,6-Biphosphate. Intermediates of glycolysis that are common to other pathways include glucose-6-phosphate (PPP, glycogen metabolism), F6P (PPP), G3P (Calvin, PPP), DHAP (PPP, glycerol metabolism, Calvin), 3PG (Calvin, PPP), PEP (C4 plant metabolism, Calvin), and pyruvate (fermentation, acetyl-CoA genesis, amino acid metabolism). After Glucose combines with phosphorous it forms more reactive compound called Glucose-6-Phosphate. BioVision provides various metabolite, enzymes and co-enzyme assay kits for your research. If there is less ATP in cell, then the process slows down and if there is need of more ATP then the rate increases. The reaction is catalyzed by the enzyme Hexokinase in the presence of Mg++ ion. These 10 steps of glycolysis process can be futher sub- divide into 2 phases- Energy requiring phase and Energy releasing phase. In this reaction one molecule of NADH is released. Final product is production of two ATP molecules. These two compounds thus formed in 4th step are isomers of each other and are convertable. Gaurab Karki ➡️Step 2,4,5,6,7,8 and 9 of Glycolysis are Reversible. Note:–After glycolysis- The presence or absence of oxygen determines whether it goes to kreb’s cycle to form ATP OR it goes to fermentation process. 3-PG ⬅️➡️ Phosphoenol     Pyruvate. Reaction 4: fructose-1,6-bisphosphate splits into 2 3-carbon molecules, one aldehyde and one ketone: dihyroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (GAP). • Anaerobic Glycolysis: It occurs when oxygen is scarce. As there are 10 steps of glycolysis, all steps are enzymatic. The latter pathway, anaerobic glycolysis, is believed to be the first process to have evolved in nature to produce adenosine triphosphate (ATP). Glycolysis takes place in the cytoplasm. Steps of Glycolysis Glycolysis is an extramitochondrial pathway and is carried by a group of eleven enzymes. Fructose-2,6 bisphosphate is potent activator of phosphofructose kinase while Fructose-1,6-bisphosphate is inhibitor of phosphofructose kinase. Addition of Phosphorous traps the Glucose inside the cell and it can no longer bind with Glucose Transport Proteins(GLUT) and cannot leave the cell. There are total 10 different glycolysis steps with enzymes starting from Glucose and ending at Pyruvate or Pyruvic acid. Helps in transport of electrones and forms ATP and forms ATP, one phosphorous atom is to. Of each other and are convertable to G3P doesn ’ t come from ATP to glucose 6-phosphate as linear! 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