Slide method 2. There are many reasons why your doctor may order a blood smear. Consequently, monitoring of laboratory performance by an external quality assessment scheme is strongly recommended. If … %PDF-1.5 Maintain even contact throughout the spreading motion. Automated slide making and staining. <>/Pattern<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> Second slide (ideally narrower than smear slide, to avoid spreading the cells over the edge) is placed on the smear slide in front of the blood spot in a … Keep practicing with these guidelines in mind, and you will be making textbook-perfect slides in no time. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. Push the blood down the patient slide with the pusher slide. Blood sample collection may take about five minutes. <>>> 3) Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. 1 In private practice, rapid evaluation of blood smears alone can identify major hematologic changes, provided the specimens are well prepared and examined systematically. 2) Use the second slide as a “spreader” slide. Meeting these parameters gives you the best opportunity to see cells as they would be in vivo and approximately quantify them. Techniques of making good bacterial smear. 2. This site uses Akismet to reduce spam. Peripheral Blood Smear. A thin blood smear can be prepared by taking a drop of blood on a grease free slide and spreading it evenly across the slide by means of a smooth edged spreader. Cover glass method 3. Spin method 7. No special preparation is required by the patient before the blood smear test. Tossed. 05 Nov 2020 by Goblusal. One is used as smear slide. Textbooks teach you to place the patient slide on the table and push the pusher slide, with the shorter edge touching the blood, away from you. The blood droplet on the right is the preferred size. Rouleaux formation is a striking feature that results from a high level of serum M-protein. endobj <> popular. 2. Then please share with your network. <>>>/Length 29>> Learn how your comment data is processed. Puncture the ball of the finger, or in infants puncture the heel. Place a drop of blood on the patient slide near the frosted edge. endobj stream Place either the long or the short edge of the pusher slide just in front of the blood drop, holding it at an approximately 30-degree angle from the patient slide. Appearance of WBC's nuclei in a well-stained peripheral blood smear microscopically. Simply trusting in yourself can help improve your slide-making skills. Smear technique. q 16 0 0 1 0 0 cm/Image6 Do Q Tossed. The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. 3 0 obj The blood smear test plays an important role in the speedy diagnosis of certain infections or diseases. An ideal slide is neither too thin nor too thick. Optimise spreading speed for length and a good feathered edge. You can control for this variation by adjusting the items listed above. However, the effectiveness and accuracy of that interpretation depend on the quality of the slide. Once blood has spread along the edge of the second slide then pull it away from the drop of blood firmly and swiftly. This keeps the edge mostly straight rather than looking like a thumbprint with a severely rounded edge. If you have any other tips or insights on making peripheral blood smears, feel free to put them in the comments! Wipe away the first drop of blood with clean gauze. Use a small inoculum (or one colony) of the microorganism from a culture plate. One of the most common types of peripheral blood slides is the wedge slide. 8 0 obj Slide technique; Cover slip technique; Automated slide making and staining; What is the procedure of preparing peripheral smear ? 22 Jan 2021 by Goblusal. The pusher slide pushes the blood down the patient slide to spread the sample out and create a monolayer of cells. 11 0 obj <>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 13 0 R/Group<>/Tabs/S/StructParents 1>> A blood smear is a blood test used to look for abnormalities in blood cells. Most starting external quality o … Aim for a blood droplet size of 4mm diameter. With the advent of hematological automated machines, complete and highly accurate hematological data and parameters like complete blood count or CBC can be obtained within a few minutes. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. Name the types of peripheral blood smear. 1 0 obj �9@�� v�.� Fixation of blood smear. The McGee Home: Master Bedroom. 13 0 obj endobj Try different ways of holding the slides to see which feels best for you. Posted in Education. This article will cover some useful tips for improving the quality of peripheral blood smears used for morphologic rather than chemical analysis. endstream endobj When I make a slide, I have confidence that it will be a quality smear, which helps me maintain a consistent speed as I push. 7. Blood Smear Technique. x�{�������! 6 0 obj 4 0 obj stream <> A high hematocrit level leads to thicker blood; a low hematocrit means thinner blood. Slide two? q 16 0 0 1 0 0 cm/Image6 Do Q As with everything, practice makes perfect. Materials 2 (75mm x 25mm) clean glass slides. It should end about two-thirds to three-fourths of the way down the slide. 1) THIN BLOOD FILM Thin PBF can be prepared from anticoagulated blood obtained by venepuncutre or from free flowing finger prick blood by any of the following three techniques : 1. Using a capillary tube collects blood from the tube and put two large drops at the center of a sterile microscopic slide. Select the finger to puncture, usually the middle or ring finger. 9 0 obj <> Ten slides later and you still don’t have a fantastic, easy to read slide. Blood Smear 6a 6b 7 The PULL technique: Take a second slide and lie the edge flat on the smear slide. Maintaining a consistent pushing speed prevents streaks on the edge of the slide as well as lines within the readable area. You can also change the angle of the pusher slide. High hematocrits occur most frequently in newborns and patients with certain cancers such as polycythemia vera. Appearance of Neutrophil's cytoplasm in a well-stained peripheral blood smear microscopically. endstream Normally, the pusher slide is held at a 30-degree angle from the patient slide, but it may be necessary to decrease that angle for patients with high hematocrits to prevent the smear from being too short and thick. 10 0 obj Collect blood in an EDTA tube and make the smears when back at the clinic. Make the thin smear starting about 1/3 from the nonfrosted end of the slide. ... Making a combined thick and think smear for mammal blood is only possible if only one smear is made per slide. You must push more slowly to ensure that the blood spreads far enough down the slide. Hello, Friends ... Leishman Stain Procedure,ideal blood smear,blood smear technique. Make the smear from fresh blood. <>>>/Length 29>> Slide one? Clinical relevance of this analysis and its interpretation are very important. These are some of the errors I see almost every student make when they first start making slides. Clean the area to be punctured with 70% alcohol; allow to dry. stream When making a wedge smear, there are two slides involved: the pusher slide and the patient slide. <> The other is pusher or the spreader slide Drop of blood (2-3 mm) in diameter: The blood can be obtained from finger Heal Microhematochrit tubes (non heparinised for EDTA or Heparinised for capillary blood) Wooden stick or glass capillary pipette Left to right: • slide 1 – perfect smear • slide 2 – smear technique interrupted in middle • slide 3 – smear was skewed • slide 4 – blood droplet too thick • slide 5 – smear … The pusher slide pushes the blood down the patient slide to spread the sample out and create a monolayer of cells. <> There are many ways to hold the slides, depending on what works best for you. endstream First, and maybe most important, when pushing the slide, the speed with which you push it needs to be consistent and smooth. 12 0 obj PDF | The process of medical diagnosis is an important stage in the study of human health. A blood smear is used when a CBC with differential shows the presence of abnormal or immature cells. Knowing the patient’s age and health status can help you determine the best way to make your slide. Thin smear; Thick smear; What are techniques for peripheral smear preparation. Allow the blood to spread along the edge of the slide. x��V�n�0��?̑`��H r��"A�.q�CЃ"3�RYJe'F��C-N���bs��7��c�����.ρ�������q΅�܂�"͡qa���00[����7����0���3�W �o���A�cI��k)YlAfDJ�g�5��������>Y���cf�~�rń�m+4�kB��Q�؛�C֛��ڡ�H��C$L(�Y����l����?.�U:�����Ӛl�u��o��w����+�.�%$\�� ������1��CZ̀�j����#�t�wT��{?cp�`�؄)�Ak'���7�Ƣp��᳙��~���%�\����TD�-�w�\���d7����os��q����K��2�,x�ro�-�c�mr�B�� Ù�J ��@�fp�� Hold the spreader slide at 30-40 degrees to achieve optimal smear length. Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… However i'm having a little trouble performing the technique to obtain the permanent preparations. 8. I hold the patient slide in my other hand the same way as the pusher slide but held perpendicular to it to give me more stability. Peripheral blood film or Peripheral blood smear or Peripheral smear examination forms an important and basic lab investigation for diagnosing a number of hematological (blood) disorders. Modified Knott’s Technique and Direct Blood Smear The modified Knott’s method is used for the concentration and identification of microfilariae, specifically the heartworm Dirofilaria immitis. 7 0 obj The quality of the blood smear depends on a proper technique and quality of the staining. Staining of blood smear. The three main blood cells that the test focuses on are: red cells, which carry oxygen throughout your body The technique for making and storing dried blood samples is given in the section “Dried Blood Samples”. A small drop of blood is placed on the midline at the end of a glass slide. Preparation of blood smear. Has this helped you? endstream 1) On one slide (the sample slide), place a small drop of blood . Thinner blood behaves exactly the opposite as blood from patients with high hematocrit levels. stream Once prepared, ... a second blood smear should also be prepared for subsequent staining by the Gram technique. 3. 2 0 obj I made a chart to highlight the changes you can make. When making a wedge smear, there are two slides involved: the pusher slide and the patient slide. Manual wedge technique 2. The peripheral blood smear may have a blue hue and show rouleaux formation of the red blood cells because of a high level of immunoglobulin (Figure 21-3). Making blood smear slides can be critically important to hematologic investigation and diagnosis. To make your slide, there are just four steps to follow: With every slide you make, there are a couple of things that you should always do to ensure the best quality. Blood smear analysis is a well known technique in medical laboratories. endobj In the end, all that matters is that the pusher slide edge is flat against the patient slide. The most common technique of blood smear preparation is called the “wedge or push” technique. Giemsa stain is commonly used when there is need to examine the Blood smear for the Parasites but is a good stain for routine examination of blood smear and used to differentiate nuclear and cytoplasmic morphology of the various cells of the blood like Platelets, RBCs.... Giemsa Staining Technique… endobj Using a larger drop size can help get enough blood to spread the desired two-thirds to three-fourths of the length of the slide. stream To avoid this, I hold the pusher slide in my dominant hand with my thumb and index finger on the short edges and use the long edge to push the blood, thus keeping me from running off at an angle. When done correctly, it should result in a uniform blood film, that gets progressively thinner. To make your slide, there are just four steps to follow: Place a drop of blood on the patient slide near the frosted edge. The healthcare professional will collect a small amount of blood in a test tube using a needle inserted into one of the veins in the patient’s arm. 4. endobj Second, let the blood spread all the way out before you start to push. Copyright © 2020 Science Squared - all rights reserved, Analytical Chemistry and Chromatography Techniques. No good either. Blood smear: slide-to-slide technique This item is available to registered subscribers only Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login A blood smear evaluates red blood cells (RBC morphology) white blood cells (WBC) and platelets to help diagnose anemia, infections or blood disorders. %���� The blood droplet on the left is too big and will result in a thick smear. As such, you need to make the opposite adjustments you would make for thicker blood. Push the second slide back until it contacts the drop of blood. That method always led to me hitting my hand or having my slide go off at an angle. Once you know how you want to hold the slides, there are a few adjustments you can make as you push the slide, depending on the quality of blood: In my experience, the main parameter that affects how the blood pushes is the hematocrit level. <> Blood smear examination also is a useful quality control measure to verify leukocytosis, leukopenia, or thrombocytopenia, especially when blood count data apparently do not “fit” the clinical picture. Agglutination of red blood cells and their decreased count in the blood smear, along with findings from t… <> Types of thin film technique. endobj Peripheral blood smear usually shows normochromic normocytic anemia. Preparation of thin blood smear. Slide three? Coverslip method 3. Push the edge of the pusher slide into the blood drop so that it spreads out completely along the pusher slide. Making a quality blood smear Although there are several techniques described for making blood smears, most people use the wedge or push technique. endobj The pathologist or a specialist will make a smear of the blood on a glass slide and observe it under the microscope for abnormalities. 5. Thicker blood spreads more slowly while thin blood spreads more quickly. Drop of blood is placed about 1-2cms from one end at the central line of slide In infants, puncture the heel. A large inoculum will result to the piling of bacteria on top of one another, thus making it difficult to get a clear image during microscopy. 5 0 obj Preparation of blood smear 6. blood smear will be made. 5. �I Ԑ�cC��)U0�� �����pG�%e]�9��. Pink to tan w/ violet/lilac granules. The end of the smear should be thin enough that it has a rainbow-like reflectiveness about it, and there should be no streaks at the very edge. They can be a useful tool to assess everything from white blood cell disorders, such as leukemias, to various types of acquired or congenital anemias, to broader organ issues like liver or kidney disease. endobj The thickness of the blood affects how quickly it spreads against the pusher slide and how far down the slide it pushes. Leishman stain procedure and Blood Smear Preparation | DLC Count. endobj What is blood smear procedure.Sometimes a nurse may be required to take a blood smear specially when there is sudden rise of body temperature and a doctor is not available.. Always start with room temperature, well-mixed, clot free, EDTA anticoagulated blood (heparin is used for some exotic species). The pusher slide should be pushed faster, you can use a smaller blood drop, and the angle can be increased to up to 45 degrees. Use clean, high-quality microscope slides. x��UKo�@�[������>��Iˣ�R F*�q[P�д�߳� yĹ������ogf�0�����j������'p�&B �0`H���u�|:�&MF���^7P=�h��j�w��(��K7'i�>M��j�GB������~'ؖl6�GR���*�e���5��E�r��ȅSTi������E�hk���؈�SoP)QQ�6z=��cu��s,zG�s�T�0�t^ATf�&�uGT�9��`p��tcp�nDǦQQW��]�6��!������?˜���Dp �� ���yH���-o�R�QN����}��Pf����tk�N�t�Ap�d�#�$Ѷ�eq|�I�LJ�`�K�.r:t5RY_��\z��ln��͋��,��R_�ZBqv�_��ʐ��|ᵂb.��p��u6���~��Mיc��,ge�4�O�I�%C�~f���L���R�7�;Bq_4�+i�^�7弮��^.`V�WO�PJ{/V�9d�͖u��L�"Ӛ��]��>Ü=ջb~�L� <> The blood smear is created by carefully extending this drop of blood in a uniform fashion with the edge of a second slide held at a 45 degrees angle to the first. Making a smear 1. When I make a slide, I know what adjustments to make as soon as I see the blood spread against the pusher slide. It must be differentiated from the non-pathogenic microfilaria of Dipetalonema reconditum (“Dipet” for short). 6. endobj